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POSITION DESCRIPTION:
Iasobio-shanghai innovation center is seeking a Research Investigator with the following duties:
?Develop new assay to address analytical characterization need for various cell therapy programs
?independently go through published literatures and design innovative experiments to find technical solutions for complex biological questions
?perform routine testing and characterization of cell therapy products using existing and developed analytical assays and immunological methods
?manage and maintain critical material inventory for different programs; document personal experimental results accurately and completely in laboratory notebook; contribute to technical documents such as test methods, reports, protocols, and internal presentations
?present study results at team or department meetings to external partners or at conferences
?contribute to technical transfer of experimental data/procedures internally and external stakeholders as appropriate; and collaborate departmentally as well as cross functionally with peers on project work as appropriate.
REQUIREMENTS:
PhD’s degree in biotechnology, immunology, cell biology or related field, plus 2 years of related experience. Prior experience must include:
?Develop and validate CAR-T generation method using viral vector or non-viral vector delivery system in support of ex vivo and in vivo delivery.
?maintain immune and tumor cell lines to develop tumor models
?Conduct ex-vivo experiments to test the efficacy of experimental CAR-T cells on tumor cells in vitro as well as in vivo
?Perform immunophenotyping using flow cytometry to assess different immune cell populations
?Characterize CAR-T function and persistence using T cell activation, cell killing (single and repeated cycle) as well as safety control by logic gating.
?Execute cytokine analysis from supernatant of CAR-T-treated tumor samples using ELISA or Luminex