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POSITION DESCRIPTION:
Iasobio-shanghai innovation center is seeking a Research Senior Associate, Cell Therapy with the following duties:
?maintain primary immune cell cultures and produce human and mouse CAR T cells.
?Plan, prioritize, and execute CAR T cell production, applying leadership qualities to facilitate a seamless workflow for experiments.
?Establish new experimental designs for CAR T cells co-cultured with other target cell types using a range of techniques and analytical method such as flow cytometry, cytotoxicity readouts, and/or gene expression.
?Produce, titer, and bank viral vectors to express CAR and other constructs in T cells.
?Collaborate with project leaders to plan experiments, analyze and interpret data, and communicate findings internally.
?Prepare study protocols, reports, and documentation.
?Work with manager and teammates to troubleshoot complex problems in creative and effective ways.
?Actively participate in the innovation and optimization of cell therapy processes to ensure the delivery of project goals.
REQUIREMENTS:
Master's degree in biotechnology, immunology, cell biology or related field, plus 3 years of related experience. Prior experience must include:
?maintain primary cell cultures, process tissue samples into single cell suspensions, cryopreserve and recover cell lines and primary immune cells
?execute CAR-T generation and related function assays including viral vector or non- viral vector generation and titration
?perform flow cytometry-based experiments, including panel optimization, gating strategy, and data analysis, including flow cytometer instruments maintenance and troubleshooting, consulting to optimize instrument setup, and assisting users to operate the instruments
?optimize in-vitro assays for enhancing T cell killing and persistence.